期刊
FOOD AND AGRICULTURAL IMMUNOLOGY
卷 26, 期 5, 页码 746-760出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09540105.2015.1027666
关键词
biotin-streptavidin enzyme-linked immunosorbent assay (BA-ELISA); diethyl phthalate (DEP); polyclonal antibody (pAb-DEP); wine
资金
- National Natural Science Foundation of China [21177082, 21477074]
- Shanghai Jiao Tong University Science, and Technology Innovation Special Fund Development Projects
An indirect competitive biotin-streptavidin enzyme-linked immunosorbent assay (BA-ELISA) has been established for the determination of diethyl phthalate (DEP) in wine samples. A highly sensitive and specific polyclonal antibody (pAb-DEP) targeting DEP was prepared. Under optimized conditions, good linearity was achieved within a range of 0.021-9.512 mu g center dot L-1. The limit of detection (IC10) was 0.0079 mu g center dot L-1 and the median inhibitory concentration (IC50) was 0.443 mu g center dot L-1. Besides, the BA-ELISA was highly selective, with low cross-reactivity values with DEP analogs (below 5%). Finally, the concentrations of DEP in wine samples ranged from 5.93 mu g center dot L-1 to 59.15 mu g center dot L-1 by BA-ELISA. Satisfactory recoveries (89.19-112.33%) and variation coefficient values (5.81-9.43%) were successfully obtained. The consistency between the results of BA-ELISA and gas chromatography-mass spectrometry (GC-MS) was 97.48%, which further confirmed that the proposed BA-ELISA immunoassay is reliable, rapid, sensitive, and accurate for monitoring DEP in wine samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据