期刊
FOOD ANALYTICAL METHODS
卷 8, 期 8, 页码 2052-2058出版社
SPRINGER
DOI: 10.1007/s12161-014-0076-4
关键词
Oxytetracycline; Screening; Fish; Dispersive liquid-liquid microextraction; Europium-sensitized luminescence
资金
- Training Program of Fujian Excellent Talents in Universities, Fujian, China
A screening protocol was developed for oxytetracycline (OTC) residue in catfish muscle based on dispersive liquid-liquid microextraction (DLLME) and europium-sensitized luminescence (ESL). The analyte was extracted in HCl-ethylenediaminetetraacetic acid (EDTA)-acetonitrile, cleaned up by DLLME in water-acetonitrile-hexane-CH2Cl2, and detected by ESL using a portable luminescence photometer. A threshold was established at T = x (2) -aEuro parts per thousand 3 sigma(2), where x (2) and sigma(2) were mean and standard deviation of ESL signals of catfish muscle samples (n = 20) spiked at 2 mu g g(-1), the tolerance set by the US Food and Drug Administration (FDA). Among 48 samples randomly spiked with OTC at 0-4 mu g g(-1), 46 were screened correctly without false negative, and 2 negatives were classified as presumptive positives. This reliable method cut usage of chlorinated solvent to 0.75 mL, reduced cost, and improved sample throughput.
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