Vc-Functionalized Fe3O4 Nanocomposites as Peroxidase-like Mimetics for H2O2 and Glucose Sensing

A simple and efficient colorimetric biosensing for hydrogen peroxide and glucose with peroxidase-like vitamin C(V-c) functionalized Fe3O4 magnetic nanoparticles(V-c/Fe3O4 MNPs) as a catalyst is reported. Compared with Fe3O4 MNPs and other catalysts, V-c/Fe3O4 MNPs exhibited superior catalytic properties. Kinetic studies indicated that vitamin C incorporated on Fe3O4 MNPs improved the affinity toward H2O2. As low as 0.29 mu mol/L H2O2 can be detected with a wide linear range of 0.5-100 mu mol/L H2O2; moreover, as low as 0.288 mu mol/L glucose can be detected with a linear range of 0.5-25 mu mol/L glucose. The detection method was highly sensitive in sensing H2O2 and glucose. The robustness of V-c/Fe3O4 MNPs rendered them suitable for wide ranging applications.