期刊
CHEMICAL COMMUNICATIONS
卷 54, 期 3, 页码 299-302出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7cc07344e
关键词
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资金
- JSPS KAKENHI [17H05839, JP16H06280]
- JST PRESTO
- World Premier International Research Center (WPI) Initiative (Japan)
- JSPS
The far-red emissive fluorescent probe CaPF-1 based on a phospha-fluorescein scaffold enables the detection of cytosolic calcium ions in living cells. The probe can be excited in the red region (lambda(abs) = 636 nm) and exhibits a sufficiently high fluorescence turn-on response in the far-red region (lambda(em) = 663 nm) upon complexation with calcium ions. The hydrophilic and anionic characteristics of this phospha-fluorescein fluorophore allowed the cytosolic localization of CaPF-1. Moreover, it was possible to visualize histamineinduced calcium oscillation in HeLa cells using CaPF-1.
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