期刊
CHEMICAL COMMUNICATIONS
卷 54, 期 57, 页码 7892-7895出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8cc02760a
关键词
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资金
- Ministry of Education, Culture, Sports, Science, and Technology (Japan) [17H03087]
- JST CREST, Japan [JPMJCR15P3]
- JSPS KAKENHI [JP15H05806]
- Grants-in-Aid for Scientific Research [17H03087] Funding Source: KAKEN
Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.
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