期刊
CHEMBIOCHEM
卷 19, 期 14, 页码 1471-1475出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201800097
关键词
mutagenesis; polymers; protein engineering; sustainable chemistry; waste disposal
资金
- Fundamental Research Funds for the central Universities
- National Key Research and Development Plan [SQ2016YFJC040104]
- National Natural Science Foundation of China [81670008, 81501787, 81502966]
Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate-binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure-guided site-directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据