期刊
CELLULAR REPROGRAMMING
卷 20, 期 1, 页码 17-26出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/cell.2017.0034
关键词
measles virus; persistent infection; human-induced pluripotent stem cells
资金
- NIH [R03 AI094164-01A1]
- Richard H. Holzer Foundation
- American Associates of the Ben-Gurion University (AAB-GU) the Philadelphia Chapter
In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles. Following MV persistent infection, BGU-iPSCs and EMF-iPSCs remain pluripotent and can differentiate in vitro into the three germ layers. This includes cells expressing the neuronal differentiation markers: NF68 and miRNA-124. Since the MV does not integrate into the cell's genome, it can be utilized as a vehicle to systematically introduce genes into iPSC, to dissect and to define factors regulating lineage differentiation.
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