期刊
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
卷 48, 期 1, 页码 361-370出版社
KARGER
DOI: 10.1159/000491742
关键词
Interleukin-8 (IL-8); Tissue engineered bone (TEB); Bone marrow mesenchymal stem cells (BMSCs); CXCR2; PI3k; Akt
资金
- Natural Science Foundation of China [81471802]
- Foundation of Southwest Hospital [SWH2015QN05]
Background/Aims: Tissue engineering bone transplantation with bone marrow mesenchymal stem cells (BMSCs) is an effective technology to treat massive bone loss, while molecular regulation of the bone regeneration processes remains poorly understood. Here, we aimed to assess the role of interleukin-8 (IL-8) in the recruitment of host cells by seeded BMSCs and in the bone regeneration. Methods: A transwell assay was performed to examine the role of IL-8/CXCR1/CXCR2/PI3k/Akt on the migration potential of hBMSCs. The in vitro chondrogenic differentiation of hBMSCs was assessed by examination of 2 chondrogenic markers. Sox9 and type 2 collagen (COL2). mBMSCs were used in tissue engineered bone (TED) with/without IL-8 implanted into bone defect area with CXCR2 or Akt inhibitors. Density and Masson staining of the regenerated bone were assessed. The chondrogenesis was assessed by expression levels of associated proteins, Sox9 and COL2, by RT-qPCR and by immunohistochemistry. Results: IL-8 may trigger in vitro migration of hBMSCs via CXCR2-mediated PI3k/Akt signaling pathway. IL-8 enhances osteogenesis in the TEB-implanted bone defect in mice. IL-8 induces chondrogenic differentiation of hBMSCs via CXCR2-mediated PI3k/Akt signaling pathway in vitro and in vivo. Conclusions: IL-8 enhances therapeutic effects of MSCs on bone regeneration via CXCR2mediated PI3k/Akt signaling pathway. (C) 2018 The Author(s) Published by S. Karger AG, Basel
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