期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 75, 期 15, 页码 2843-2856出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-018-2764-5
关键词
Spinal cord; Dental stem cells; Inflammation; Oligodendrocyte progenitor cells; Differentiation
资金
- Universite Catholique de Louvain (FSR)
- International Foundation for Research in Paraplegia (IRP)
- MRC [MR/N022556/1, MR/M020827/1] Funding Source: UKRI
- Action Medical Research [2318] Funding Source: researchfish
- Medical Research Council [MR/N022556/1, MR/M020827/1] Funding Source: researchfish
Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-alpha can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-alpha expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.
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