期刊
CELL STRESS & CHAPERONES
卷 23, 期 4, 页码 791-796出版社
SPRINGER
DOI: 10.1007/s12192-017-0869-7
关键词
Albino Channa argus; Heat shock protein 60; Temperature stress; Gene expression
类别
资金
- Science and Technology Planning Project of Guangdong Province [2017A020225035, 2016A020210141]
- Produce-learn-research Project of Guangdong Province [2011B090400270]
- Fund Fostering Talents for Young Scholars of South China Agricultural University [201707 N025]
- Talent introduction special funds of South China Agricultural University
- Scientific Research Staring Foundation for Young Scholars of College of Marine Sciences
The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In the present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. The cDNA was 2462 bp, containing an open reading frame (ORF) encoding a 575-amino-acids polypeptide of 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 was highly similar with other HSP60s, and three conserved amino acid blocks and characteristic motifs or domains defined as HSP60 protein family signatures. Genomic DNA analysis showed that AcaHSP60 had ten exons in the coding region (from 94 to 336 bp). Changes in AcaHSP60 gene expression profiles in albino C. argus experimentally exposed to different temperature stress (8.5, 26, and 37 A degrees C) was investigated. Quantitative real-time PCR and western blot analysis revealed that tissue-specific AcaHSP60 expressions were in the spleen, muscle, liver, kidney, heart and brain. Expression was highly significantly stimulated after heat shock (37 A degrees C), but showed no significant differences after cold treatment (8.5 A degrees C) except in the brain. In summary, these results showed that AcaHSP60 was significantly tissue specific and indicate that AcaHSP60 expression might be sensitive to thermal resistance in albino C. argus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据