期刊
CELL STEM CELL
卷 22, 期 1, 页码 50-+出版社
CELL PRESS
DOI: 10.1016/j.stem.2017.11.004
关键词
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资金
- Smoking Research Foundation
- Core Research for Evolutional Science and Technology (CREST) from the Japan Agency for Medical Research and Development (AMED)
- KAKENHI [26112502, 15K10657, 17H04335]
- Grants-in-Aid for Scientific Research [26112502, 15K10657, 17K19709, 17H04335] Funding Source: KAKEN
Trophoblast cells play an essential role in the interactions between the fetus and mother. Mouse trophoblast stem (TS) cells have been derived and used as the best in vitro model for molecular and functional analysis of mouse trophoblast lineages, but attempts to derive human TS cells have so far been unsuccessful. Here we show that activation of Wingless/Integrated (Wnt) and EGF and inhibition of TGF-beta, histone deacetylase (HDAC), and Rho-associated protein kinase (ROCK) enable long-term culture of human villous cytotrophoblast (CT) cells. The resulting cell lines have the capacity to give rise to the three major trophoblast lineages, which show transcriptomes similar to those of the corresponding primary trophoblast cells. Importantly, equivalent cell lines can be derived from human blastocysts. Our data strongly suggest that the CT- and blastocyst-derived cell lines are human TS cells, which will provide a powerful tool to study human trophoblast development and function.
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