期刊
CELL STEM CELL
卷 22, 期 5, 页码 726-+出版社
CELL PRESS
DOI: 10.1016/j.stem.2018.04.005
关键词
-
资金
- Pew Charitable Trusts
- NIDCR-Interdisciplinary Training Program [T32HD075735]
- University Unit Programme Grant from the UK Medical Research Council [U127527202]
- NIH/NHBLI [R01 HL133120]
- NIH/NIAMS [R01 AR063724, R01 AR069078]
- Tisch Cancer Institute
- MRC [MC_PC_U127527202] Funding Source: UKRI
Polycomb repressive complexes (PRCs) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that, despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification, whereas PRC1 catalytic activity is dispensable. Further dissection demonstrated that both canonical and non-canonical PRC1 complexes bind to repressed genes, marked by H2AK119ub and PRC2-mediated H3K27me3. Interestingly, loss of canonical PRC1, PRC1 catalytic activity, or PRC2 leads to expansion of mechanosensitive Merkel cells in neonatal skin. Non-canonical PRC1 complexes, however, also bind to and promote expression of genes critical for skin development and SC formation. Together, our findings highlight PRC1's diverse roles in executing a precise developmental program.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据