期刊
CELL HOST & MICROBE
卷 24, 期 1, 页码 120-+出版社
CELL PRESS
DOI: 10.1016/j.chom.2018.06.002
关键词
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资金
- NIH [GM105456, GM103574, GM118159, AI124275]
- Burroughs Wellcome Fund
- Swiss National Science Foundation [P2EZP3_178482, P2EZP3_162256]
- European Molecular Biology Organization [ALTF 670-2016]
- Swiss National Science Foundation (SNF) [P2EZP3_178482, P2EZP3_162256] Funding Source: Swiss National Science Foundation (SNF)
In the mammalian gut, bacteria compete for resources to maintain their populations, but the factors determining their success are poorly understood. We report that the human gut bacterium Bacteroides thetaiotaomicron relies on the stringent response, an intracellular signaling pathway that allocates resources away from growth, to survive carbon starvation and persist in the gut. Genome-scale transcriptomics, C-13-labeling, and metabolomics analyses reveal that B. thetaiotaomicron uses the alarmone (p) ppGpp to repress multiple biosynthetic pathways and upregulate tricarboxylic acid (TCA) cycle genes in these conditions. During carbon starvation, (p) ppGpp triggers accumulation of the metabolite alpha-ketoglutarate, which itself acts as a metabolic regulator; alpha-ketoglutarate supplementation restores viability to a (p) ppGpp-deficient strain. These studies uncover how commensal bacteria adapt to the gut by modulating central metabolism and reveal that halting rather than accelerating growth can be a determining factor for membership in the gut microbiome.
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