期刊
CELL
卷 173, 期 5, 页码 1204-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2018.03.008
关键词
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资金
- DOD PCRP [PC150946]
- AACR-Bristol-Meyer Squib Oncology Fellowship in Clinical Cancer Research
- NIH-NCI [1K08CA175154]
- Burroughs Wellcome Fund Career Award for Medical Scientists
- V Foundation Scholar Award
- AACR-Bayer NextGen Grant for Transformative Cancer Research
- Swedish Foundations' Starting Grant (SFSG)
- StemTherapy
- Swedish Research Council (Vetenskapsradet) [E0236201]
- Swedish Cancer Society (Cancerfonden) [CAN2014/572]
- NATIONAL CANCER INSTITUTE [K08CA175154] Funding Source: NIH RePORTER
Pseudouridylation (J) is the most abundant and widespread type of RNA epigenetic modification in living organisms; however, the biological role of J remains poorly understood. Here, we show that a J-driven posttranscriptional program steers translation control to impact stem cell commitment during early embryogenesis. Mechanistically, the J writer'' PUS7 modifies and activates a novel network of tRNA-derived small fragments (tRFs) targeting the translation initiation complex. PUS7 inactivation in embryonic stem cells impairs tRF-mediated translation regulation, leading to increased protein biosynthesis and defective germ layer specification. Remarkably, dysregulation of this posttranscriptional regulatory circuitry impairs hematopoietic stem cell commitment and is common to aggressive subtypes of human myelodysplastic syndromes. Our findings unveil a critical function of J in directing translation control in stem cells with important implications for development and disease.
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