Soluble soybean polysaccharide/TiO2 bionanocomposite film for food application

In the current study, a set of biodegradable soybean polysaccharide (SSPS) nanocomposites containing different ratios of TiO2 nanoparticles was characterized as new packaging system. X-ray diffraction (XRD) measurement showed that the crystalline structure of the TiO2 nanoparticles remained intact in the polysaccharide matrix and the surface of nanocomposites containing 1-3% TiO2 was observed morphologically uniform under scanning electron microscopy (SEM). Dynamic mechanical thermal analysis revealed that the magnitude of storage modulus was 3.62-fold higher in SPSS/TiO2 nanocomposites containing 7 wt.% of TiO2 than control SSPS indicating improvement in the physical properties of the film supposed to be utilized for food packaging. With respect to the concern over the safety of these nanocomposites, inductively coupled plasma-optical emission spectroscopy (ICP-OES) showed that no TiO2 was detected in bread samples covered by SSPS/TiO2 film and stored for 6 months. Similarly, the nanocomposite films only released a minuscule amount (21.05 +/- 0.054 ppm) of TiO2 in water. TiO2 nanoparticles were found in the plasma membrane of epithelial cell line after long-term exposure (10-day) of these cells to large amounts of the free nanoparticles. SSPS/TiO2 nanocomposites showed excellent antimicrobial activity against Staphylococcus aureus PTCC 1431 (ATCC 25923), while neither anticancerous nor pro-cancerous activity was observed for these nanocomposites denoting their neutrality with respect to cancer suppression or progression in gastrointestinal tract. In conclusion, SSPS/TiO2 nanocomposites could be a promising packaging system for food industries' objective regarding their physical characteristics, low rate of Ti transition, and low health risk.