期刊
CARBOHYDRATE POLYMERS
卷 182, 期 -, 页码 207-214出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.11.022
关键词
Polysaccharide; Abalone; Bone morphogenetic protein; Chondroitin sulfate; Heparin
资金
- National Key Research and Development Program of China [2017YFD0400203]
- National Natural Science Foundation of China [31301431, 31501439]
- Public Science and Technology Research Funds Projects of Ocean [201505029]
- Dalian High-level Talents Innovation and Entrepreneurship Project [2016RQ061]
Bone morphogenic protein-2 (BMP-2) is known to promote osteogenesis. To find novel adjuvants to enhance the activity of BMP-2, the present study investigated the structure BMP-2-induced osteogenic activity of a water-soluble polysaccharide from the gonad of pacific abalone (Haliotis discus hannai Ino) named AGSP. Through analysis of aldobiouronic acids released from AGSP, monosaccharide composition comparison of AGSP and its reduced product, and methylation analysis and NMR analysis of AGSP and its desulfated derivative, the main structure residue of AGSP was determined as -> 3)-GlcA(1 -> 3)-Gal(1 -> with sulfated branches comprised of prevelant Gal and minor Glc, and -> 4)-beta-GlcA(1 -> 2)-alpha-Man(1 -> residue was also found. AGSP possessed a sulfate content of 12.4% with a relative molecular weight of 6.6 kDa. AGSP strengthened alkaline phosphatase activity induced by BMP-2 in a dose dependent manner at 10-200 mu g/mL with 425% enhancement being observed at 200 mu g/mL, indicating AGSP could be an adjuvant candidate to enhance osteogenic activity of BMP-2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据