期刊
CARBOHYDRATE POLYMERS
卷 196, 期 -, 页码 246-253出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2018.05.037
关键词
Neutral polysaccharides; Epimedium koreanum Nakai; Structure; Antioxidant activity
资金
- National Foundation of China [31700703]
- Jilin Provincial Administration of traditional Chinese Medicine grant [2017179]
- Science and Technology Development Plan Project of Jilin Province [20170204010YY]
The structure and antioxidant activity of a neutral polysaccharide (named as EFPN-1) from Epimedium koreanum Nakai were studied. Based on the results from high performance gel permeation chromatography, methylation GC-MS and 1D/2D nuclear magnetic resonance spectroscopy, EFPN-1 was a branched polysaccharide with a molecular weight of 21.9 kD and containing over 60% of glucose. Its backbone was composed of (1 -> 4)-beta-D-Glcp and (1 -> 3)-beta-D-Galp, branched partially at O-6 of both residues, with a degree of branching of 40.4% and terminated mainly with Ara and Gal, as well as trace amount of Gal. Antioxidant tests showed that EFPN-1 exhibited a strong capacity on DPPH and hydroxyl radical scavenging, ferrous ion chelation and reducing antioxidant powder. EFPN-1 has potential to be developed as a natural antioxidant.
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