Fabrication of chitosan-mesoporous silica SBA-15 nanocomposites via functional ionic liquid as the bridging agent for PPL immobilization

A nanocomposite (SBA-CIL-CS) which was combined mesoporous silica SBA-15 material with chitosan via ionic liquid as the bridging agent was successfully fabricated. The morphology and structure of the nanocomposite were characterized in detail with transmission electron microscope, fourier transform infrared spectroscopy, thermogravimetric analysis and nitrogen adsorption-desorption techniques. SBA-CIL-CS was investigated as an efficient support for immobilization of porcine pancreas lipase (PPL) and possessed high immobilization efficiency. The properties of immobilized enzyme (SBA-CIL-CS-PPL) such as activity, stability and reusability have been significantly improved, and a preferable pH and temperature tolerance were obtained as well. Results demonstrated the inorganic-organic nanocomposite could be used as an ideal support for enzyme immobilization.