期刊
CANCER LETTERS
卷 420, 期 -, 页码 168-181出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2018.02.013
关键词
Ovarian cancer; EGFR; HER-2; c-MET; 3D cell culture; Targeted inhibitors; Ascites
类别
资金
- government of Iraq
- Ovarian Cancer Research Foundation (OCRF), Melbourne, Australia
Ovarian cancer patients in the advanced stages of the disease show clinical ascites, which is associated with a poor prognosis. There is limited understanding of the effect of ascitic fluid on ovarian cancer cells and their response to anticancer drugs. We investigated the antitumour effects of EGFR/Her-2 (canertinib) and c-Met (PHA665752) inhibitors in a 3D cell model of three ovarian cancer lines. Single and combined inhibitor treatments affected cell growth of OVCAR-5 and SKOV-3 cell lines but not OV-90 cell line. Growth reduction was correlated with the down expression of PCNA, EGFR, HER-2, c-MET, ERK and AKT and their phosphorylation status in cells in growth factor supplemented media. However, these effects were not re-producible in OVCAR-5 and SKOV-3 cell lines when they were exposed to ascitic fluid obtained from three ovarian cancer patients. Serum albumin and protein components in the ascitic fluids may reduce the cellular uptake of the inhibitors. (C) 2018 Elsevier B.V. All rights reserved.
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