期刊
CANCER LETTERS
卷 423, 期 -, 页码 1-8出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2018.02.039
关键词
Metastatic colorectal cancer; Circulating tumor cells; microRNA; Chemotherapy
类别
资金
- National Medical Research Council (NMRC) Clinician New Investigator Grant (CNIG) [NMRC/CNIG/1119/2014]
- National University Health System (NUHS) Bench to Bedside Grant [NUHSRO/2014/019/BBP/04]
We evaluated the changes in CTC count and CTC-associated miRNAs during the course of chemotherapy in patients with metastatic colorectal cancer. Blood samples were collected from 9 metastatic colorectal cancer patients prior to chemotherapy and at every other chemotherapy session during the course of treatment. CTCs were isolated and enumerated using a size-exclusion method (CellSievo, Singapore). CTC-associated miRNAs were isolated using a paper-based, partitioning method, and analyzed using reverse transcription quantitative real-time PCR (MiRXES, Singapore). CTC count trends generally correlated with disease progression defined by radiological measurements and trends in carcinoembryonic antigen (CEA) levels; hence CTC counts may be useful in cases where CEA is not elevated. CTC-associated miRNAs identified were miR-15b, miR-16, miR-19a, miR-21, miR-25, miR-30d, miR-126, miR-185, miR-221, miR-222, and miR-324-5p. The expression of CTC-associated miRNAs did not appear to correlate with CTC count and exhibited inter-individual heterogeneity. This pilot study suggests that analysis of CTC changes during the course of treatment may be useful in monitoring response to therapy in metastatic colorectal cancer. (C) 2018 Published by Elsevier B.V.
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