期刊
CALCIFIED TISSUE INTERNATIONAL
卷 103, 期 2, 页码 227-236出版社
SPRINGER
DOI: 10.1007/s00223-018-0410-8
关键词
MicroRNA-218; Mesenchymal stem cells; Osteogenesis; Femur fracture
资金
- Research Grants Council of the Hong Kong SAR, China [14119115, 14160917, N_CityU102/15, T13-402/17-N]
- National Natural Science Foundation of China [81772404, 81430049, 81772322]
- Hong Kong Innovation Technology Commission Funds [ITS/UIM-305]
- SMART program, Lui Che Woo Institute of Innovative Medicine
As a regulator of osteogenesis, microRNA-218 (miR-218) is reported to promote osteogenesis of mesenchymal stem cells (MSCs). However, the in vivo osteogenic effect of miR-218 remains elusive. In this study, miR-218 was confirmed to promote osteogenic differentiation of MSCs by stimulating the alkaline phosphatase activity, calcium nodule formation, and osteogenic marker gene expression. For in vivo study, the miR-218-overexpressing BMSCs were locally administrated into the fracture sites in a femur fracture mouse model. Based on the X-rays, micro-computed tomography, mechanical testing, histology, and immunohistochemistry examinations, miR-218 overexpression improved new bone formation and accelerated fracture healing. These findings suggest that miR-218 may be a promising therapeutic target for bone repair in future clinical applications.
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