期刊
BRITISH POULTRY SCIENCE
卷 59, 期 3, 页码 308-317出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/00071668.2018.1454585
关键词
Aspirin administration; chicken myocardial cells; heat stress; Hsp90; expression; inhibition of apoptosis
资金
- National Natural Science Foundation of China [31372403]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Research Innovation Program of Graduate Student in Jiangsu Province [KYLX15-0536]
- Natural Science Foundation of the Jiangsu Province [BK20140107]
- Sino-German Agricultural Cooperation Project of the Federal Ministry of Food, the Agriculture and Consumer Production, Berlin, Germany
- open project from Jiangsu Centre of Modern Animal Husbandry and New Veterinary Drug Engineering Technology
1. This experiment investigated the anti-apoptosis effects and the mechanism of aspirin action in the heat shock response of chicken myocardial cells in vivo, via changes in the heat stress (HS) protein Hsp90 and the rate of apoptosis. Broiler chickens were administered aspirin (1 mg/kg body weight) 2 h before exposure to HS, and then exposed to 40 +/- 1 degrees C for 0, 1, 2, 5, 7, 10, 15 and 24 h. 2. The induction and consumption of the HS factor heat shock factor (HSF)-1, and reductions of HSF-2 and HSF-3 induced by HS led to a delay in Hsp90 expression. HSF-1, 2 and 3 regulation of hsp90 expression in turn inhibited the synthesis and activation of protein kinase beta (Akt), which resulted in a significant increase in caspase-3 at 2 and 10 h, caspase-9 from 1 to 7 h (except at 5 h), and the heat stressed apoptosis of they myocardial cells. 3. Administration of aspirin changed the expression patterns of HSF-1, 2 and 3 such that expression of Hsp90 protein was significantly upregulated (by 2.3-4.1 times compared with that of the non-treated cells). The resultant increase in Akt expression and activation, compared with the HS group, inhibited caspase-3 and caspase-9 activities and reduced the myocardial cells apoptosis rate (by 2.14-2.56 times). 4. Aspirin administration could inhibit heat-stressed apoptosis of myocardial cells;in vivo and may, be closely associated with its promotion of HS response of chicken hearts, especially Hsp90 expression.
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