期刊
BREEDING SCIENCE
卷 68, 期 2, 页码 168-176出版社
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.17085
关键词
heat stress; adzuki; Vigna angularis; ONSEN; retrotransposon
资金
- Suharakinennzaidann, Cooperative Research Grant of the Plant Transgenic Design Initiative, Gene Research Center, the University of Tsukuba
- Joint Research Program of Arid Land Research Center, Tottori University [28C2002]
- MEXT-Supported Program for the Strategic Research Foundation at Private Universities
- [JP15H05960]
- Grants-in-Aid for Scientific Research [17K19361] Funding Source: KAKEN
In plants, several transposable elements are conserved across species. We found a homolog of ONSEN, which is a heat-activated retrotransposon originally isolated from Arabidopsis thaliana, in Vigna. The ONSEN-like elements (VaONS) were detected in all the analyzed Japanese accessions of Vigna angularis (adzuki bean) by Southern blot analysis. However, VaONS sequences were observed to be polymorphic in the different accessions. Interestingly, extrachromosomal DNA (ecDNA) was detected in some accessions of adzuki bean, indicating the conserved heat-activation of VaONS. Furthermore, we successfully induced retrotransposition of VaONS in adzuki plant regenerated through callus. Findings of our study should provide a new tool for molecular breeding of adzuki bean.
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