期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 49, 期 2, 页码 362-369出版社
SOC BRASILEIRA MICROBIOLOGIA
DOI: 10.1016/j.bjm.2017.04.013
关键词
Rumex gmelini Turcz (RGT); Endophytic fungi; Aspergillus sp.; Seedlings; Bioactive secondary metabolites
类别
资金
- National Natural Science Foundation of China [30930700]
- Natural ScienceFoundation of Heilongjiang Province [C2016053, QC2009C31]
Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz ( RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 x 10(4) mL(-1), after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
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