期刊
BRAIN PATHOLOGY
卷 28, 期 6, 页码 875-888出版社
WILEY
DOI: 10.1111/bpa.12587
关键词
Lewy bodies; Lewy body dementia; Parkinson's disease; alpha-synuclein; synapsin III
资金
- Fondazione Cariplo [2014-0769]
- University of Brescia (BIOMANE)
- Michael J. Fox Foundation for Parkinson's Research, NY [10742]
- Parkinson's UK, a charity registered in England and Wales
- Fondazione Camillo Golgi, Brescia
- Parkinson's UK, a charity registered in Scotland
Lewy bodies (LB) and Lewy neurites (LN), which are primarily composed of alpha-synuclein (alpha-syn), are neuropathological hallmarks of Parkinson's disease (PD) and dementia with Lewy bodies (DLB). We recently found that the neuronal phosphoprotein synapsin III (syn III) controls dopamine release via cooperation with alpha-syn and modulates alpha-syn aggregation. Here, we observed that LB and LN, in the substantia nigra of PD patients and hippocampus of one subject with DLB, displayed a marked immunopositivity for syn III. The in situ proximity ligation assay revealed the accumulation of numerous proteinase K-resistant neuropathological inclusions that contained both alpha-syn and syn III in tight association in the brain of affected subjects. Most strikingly, syn III was identified as a component of alpha-syn-positive fibrils in LB-enriched protein extracts from PD brains. Finally, a positive correlation between syn III and alpha-syn levels was detected in the caudate putamen of PD subjects. Collectively, these findings indicate that syn III is a crucial alpha-syn interactant and a key component of LB fibrils in the brain of patients affected by PD.
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