期刊
RSC ADVANCES
卷 6, 期 2, 页码 1268-1278出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ra18424j
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资金
- CSIR [02(2490)/11/EMR-II]
- DST, New Delhi [SR/S1/IC-20/2012]
A novel 3-hydroxymethyl-5-methylsalicylaldehydenaphthyl-hydrazone (H(3)SAL-NH) exhibits ESIPT behaviour due to proton transfer from the phenolic OH group to the azomethine N atom in the excited state. Through this ESIPT behaviour together with cis-trans isomerization of the azomethine group, the free ligand becomes very weakly fluorescent. However, in the presence of Zn2+ and Al3+ the ESIPT and isomerization are blocked due to coordination to the metal ions thereby causing turn on fluorescence for Al3+ and Zn2+. Moreover, Zn2+ can easily be displaced from the [H(2)SAL-NH-Zn2+] complex by Al3+ thereby enhancing the differential selectivity for Al3+ over Zn2+. This probe was found to be selective for Al3+ over Zn2+ in the presence of Na(2)H(2)EDTA, under both intra-and extracellular conditions. The LODs for Zn2+ and Al3+ were determined by 3 sigma methods and were found to be 3.1 nM and 0.92 nM, respectively. Thus, the differentially selective turn-on fluorescence behaviour of H3SAL-NH for Zn2+ and Al3+ is based on the combined blocking of ESIPT and C=N isomerization, and a chelation-enhanced fluorescence (CHEF) effect. The coordination modes of the complexes were investigated through spectroscopic and computational studies. H3SAL-NH also exhibits good photostability and very low cytotoxicity and is useful for fluorescence imaging of Zn2+ and Al3+ ions in live HepG2 cells.
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