期刊
BMC BIOLOGY
卷 16, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12915-018-0545-0
关键词
CRISPR; Guide library; Cas9; Cpf1; Off-target; On-target; Deletion scan
类别
资金
- Paul G. Allen Family Foundation
- NIH Directors Pioneer Award [DP1HG007811]
- NIH/NHLBI [T32HL007312]
Background: Genome-wide knockout studies, noncoding deletion scans, and other large-scale studies require a simple and lightweight framework that can quickly discover and score thousands of candidate CRISPR guides targeting an arbitrary DNA sequence. While several CRISPR web applications exist, there is a need for a high-throughput tool to rapidly discover and process hundreds of thousands of CRISPR targets. Results: Here, we introduce FlashFry, a fast and flexible command-line tool for characterizing large numbers of CRISPR target sequences. With FlashFry, users can specify an unconstrained number of mismatches to putative off-targets, richly annotate discovered sites, and tag potential guides with commonly used on-target and off-target scoring metrics. FlashFry runs at speeds comparable to commonly used genome-wide sequence aligners, and output is provided as an easy-to-manipulate text file. Conclusions: FlashFry is a fast and convenient command-line tool to discover and score CRISPR targets within large DNA sequences.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据