期刊
VETERINARY RESEARCH
卷 47, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13567-016-0311-7
关键词
-
资金
- Ministry of Agriculture, Food and Rural Affairs [iPET112012-3]
- MSIP [2014R1A2A2A01007291]
- BK21 PLUS and Research Institute for Veterinary Science, Seoul National University (Seoul, South Korea)
Brucellosis is a clinically and economically important disease. Therefore, eradication programs of the disease have been implemented in several countries. One hurdle in these programs is the detection of infected animals at the early stage. Although the protein antigens as diagnostic antigens have recently received attention, the exact mechanisms at the beginning of immune responses are not yet known. Therefore, genes encoding five B. abortus cellular proteins were cloned and the expressed recombinant proteins were purified. The expression of several cytokine genes (IL-1 beta, IL-4, IL-6, IL-12p40, IFN-gamma, TNF-alpha, and iNOS) was analyzed in bovine peripheral blood mononuclear cells (bPBMC) after stimulation with the recombinant proteins. Three apoptosis-related genes, Bax, Bcl-2, and TLR4, were also included in the analysis to find out the adverse effects of the proteins to the cells. Each protein induced different patterns of cytokine expression depending on the stimulation time and antigen dose. Expression of IL-6, IL-12p40, and IFN-gamma was induced with all of the proteins while IL-1 beta, IL-4, TNF-alpha, and iNOS gene expression was not. Expression of apoptosis-related genes was not altered except TLR4. These results suggest that the cellular antigens of B. abortus induce both humoral and cellular immunity via the production of IL-6, IL-12p40, and IFN-gamma in bPBMC without exerting any adverse effects on the cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据