期刊
BIOTECHNOLOGY PROGRESS
卷 34, 期 3, 页码 692-701出版社
WILEY
DOI: 10.1002/btpr.2612
关键词
detachable three-dimensional tissue; heating centrifuge; multilayered cell sheets; temperature-responsive culture dish
资金
- Adaptable and Seamless Technology transfer Program through target driven R&D (A-STEP) from Japan Agency for Medical Research and Development (AMED), Japan [JP17im0302706]
- Nihon Kohden Corporation
Confluent cultured cells on a temperature-responsive culture dish can be harvested as an intact cell sheet by decreasing temperature below 32 degrees C. A three-dimensional (3-D) tissue can be fabricated by the layering of cell sheets. A resulting 3-D multilayered cell sheet-tissue on a temperature-responsive culture dish can be also harvested without any damage by only temperature decreasing. For shortening the fabrication time of the 3-D multilayered constructs, we attempted to layer cell sheets on a temperature-responsive culture dish with centrifugation. However, when a cell sheet was attached to the culture surface with a conventional centrifuge at 22-23 degrees C, the cell sheet hardly adhere to the surface due to its noncell adhesiveness. Therefore, in this study, we have developed a heating centrifuge. In centrifugation (55g) at 36-37 degrees C, the cell sheet adhered tightly within 5 min to the dish without significant cell damage. Additionally, centrifugation accelerated the cell sheet-layering process. The heating centrifugation shortened the fabrication time by one-fifth compared to a multilayer tissue fabrication without centrifugation. Furthermore, the multilayered constructs were finally detached from the dishes by decreasing temperature. This rapid tissue-fabrication method will be used as a valuable tool in the field of tissue engineering and regenerative therapy. (c) 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:692-701, 2018
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