期刊
BIOTECHNOLOGY LETTERS
卷 40, 期 7, 页码 1029-1036出版社
SPRINGER
DOI: 10.1007/s10529-018-2556-x
关键词
Evaporation; Fed batch; GS-CHO cells; Miniature shaken bioreactor; Reproducibility
资金
- UK Engineering and Physical Sciences Research Council (EPSRC) Industrial Doctorate Training Centre (IDTC) in Bioprocess Engineering Leadership [EP/G034656/1]
- Applikon Biotechnology
Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. A correlation between evaporation and the concentration of Na+ was found (R-2 = 0.95) when using the 24-well-based miniature bioreactor system (micro-Matrix) for a batch culture with GS-CHO. Based on these results, a method was developed to counteract evaporation with periodic water additions based on measurements of the Na+ concentration. Implementation of this method resulted in a reduction of the relative liquid loss after 15 days of a fed-batch cultivation from 36.7 +/- 6.7% without volume corrections to 6.9 +/- 6.5% with volume corrections. A procedure was established to indirectly measure evaporation through a correlation with the level of Na+ ions in solution and deriving a simple formula to account for liquid losses.
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