期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 115, 期 11, 页码 2817-2827出版社
WILEY
DOI: 10.1002/bit.26793
关键词
gut-liver chip; hepatic steatosis; organ-on-a-chip
资金
- National Research Foundation of Korea [2016R1D1A1B03934710]
- Korea Food Research Institute [E0121705]
- Ministry of Trade, Industry and Energy of Korea [10050154]
- Hongik University Research Fund
- Establishment of Infrastructure for Industrialization of Korean Useful Microbes [R0004073]
- Korea Evaluation Institute of Industrial Technology (KEIT) [R0004073] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2016R1D1A1B03934710] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not recapitulate the complex process of absorption and metabolism of digestive lipids. Here, we introduce a gut-liver chip, which mimics the gut absorption and hepatic metabolism in a microfluidic chip. Absorption of fatty acids through gut layer and subsequent deposition within liver cells was demonstrated. Tumor necrosis factor-, butyrate, and -lipoic acid were chosen as model molecules that can affect hepatic steatosis via different mechanisms, and their effects were evaluated. Our results suggest that the gut-liver chip can mimic the absorption and accumulation of fatty acids in the gut and the liver.
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