期刊
BIOTECHNOLOGY ADVANCES
卷 36, 期 3, 页码 641-665出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2018.01.006
关键词
CRISPR-Cas; Yeasts; Metabolic engineering; Synthetic biology; Expression optimization; Saccharomyces cerevisiae; Schtzosaccharomyces pombe; Yarrowia lipolytica; Pichla pastoris; Candida albicans/glabrata
资金
- European Union (EU) project ROBOX under EU's Horizon Programme Research and Innovation actions [635734, H2020-LEIT B10-2014-1]
- MSMT [20-SVV/2017]
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Mettle pastoris syn. Komagataella phaffii, Kluyveromyces lactic, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.
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