期刊
BIOTECHNOLOGY ADVANCES
卷 36, 期 3, 页码 784-797出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2018.01.010
关键词
Algae; Carbon; Deuterium; Magnesium; Nitrogen; Oxygen; Raman spectroscopy; Selenium; Stable isotopes; Sulfur
资金
- Grant Agency of the Czech Republic [17-06264S]
Stable isotopes are used in wide fields of application from natural tracers in biology, geology and archeology through studies of metabolic fluxes to their application as tracers in quantitative proteomics and structural biology. We review the use of stable isotopes of biogenic elements (H, C, N, O, S, Mg, Se) with the emphasis on hydrogen and its heavy isotope deuterium. We will discuss the limitations of enriching various compounds in stable isotopes when produced in living organisms. Finally, we overview methods for measuring stable isotopes, focusing on methods for detection in single cells in situ and their exploitation in modern biotechnologies.
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