期刊
BIOSENSORS & BIOELECTRONICS
卷 102, 期 -, 页码 27-32出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.10.051
关键词
Paper-based lateral flow device; Enzyme-linked immunosorbent assay; Wax-printing method; Alpha-fetoprotein; Point-of-care testing
类别
资金
- Thailand Research Fund (TRF) through International Research Network [PHD58W0001]
- Thailand Research Fund through Research Team Promotion Grant [RTA6080002]
- 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) [GCUGR1125603023D]
In this study, a novel wax-printed paper-based lateral flow device has been developed as an alternative approach for an automated and one-step enzyme-linked immunosorbent assay (ELISA). The design pattern consisted of a non-delayed channel, a wax-delayed channel, a test zone and a control zone. This system was easily fabricated on a nitrocellulose membrane using a wax-printing method and then baked in an oven at 100 degrees C for 1 min. The four barriers of the wax-delayed channel could delay the flow time for 11 s compared to the flow time of the non delayed channel. To use the device under optimal conditions, alpha-fetoprotein (AFP) was detected at a limit of detection of 1 ng mL(-1) and assessed with the naked eye within 10 min. A colorimetric intensity was also measured using a smart phone and computer software at a linear range of 0.1-100 ng mL(-1) with a good correlation. Furthermore, the proposed device was successfully applied to detect AFP in human serum. Therefore, the wax-printing demonstrates a user-friendly, easy and quick method for the fabrication of the device, which could be used as a one-step, portable, disposable, low-cost, simple, instrument-free and point-of-care device for the automated ELISA.
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