期刊
BIOSENSORS & BIOELECTRONICS
卷 117, 期 -, 页码 810-817出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.07.014
关键词
Catecholamine; Dopamine; Molecularly imprinted polymer; Field-effect transistor; Surface-initiated atom transfer radical polymerization
类别
资金
- New Energy and Industrial Technology Development Organization (NEDO) under the Ministry of Economy Trade and Industry (METI) of Japan
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
We report a well-designed biointerface enabling the selective and quantitative detection of dopamine (DA) using a potentiometric biosensor. To enhance the detection selectivity of DA, a DA-templated molecularly imprinted polymer (DA-MIP) was synthesized on the extended Au gate electrode of a field-effect transistor (FET) biosensor. For a quantitative DA analysis, the thickness of the DA-MIP was controlled to ca. 60 nm by surface-initiated atom transfer radical polymerization (SI-ATRP). In this process, the DA-MIP was copolymerized with vinyl-phenylboronic acid (vinyl-PBA), inducing molecular charges at the biointerface of the FET gate electrode. These charges were generated by the diol-binding between PBA and dopamine (a catecholamine), and were directly detected as a change in surface potential. In fact, the surface potential at the gate of the DA-MIP-coated FET responded significantly to DA added at concentrations ranging from 40 nM to 20 mu M, whereas that of a non imprinted polymer (NIP)-coated FET hardly changed over this range. Moreover, by measuring the kinetic parameters and electrochemical properties of well-designed devices with various added catecholamines, we confirmed that the DA-MIP-coated FET biosensor selectively and quantitatively detects DA.
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