期刊
BIOSENSORS & BIOELECTRONICS
卷 99, 期 -, 页码 209-215出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.051
关键词
Optical fiber; 3D DNA origami nanorobot; Switchable nanodevice; Genosensor
类别
资金
- NTU-HUJ-BGTJ Nanomaterials for Energy and Water Management Programme under the Campus for Research Excellence and Technological Enterprise (CREATE)
- National Research Foundation, Prime Minister's Office, Singapore
- EPSRC [EP/J004111/2, EP/N031962/1]
- EPSRC [EP/N031962/1, EP/J004111/2] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/N031962/1, EP/J004111/2] Funding Source: researchfish
In the quest of greater sensitivity and specificity of diagnostic systems, one continually searches for alternative DNA hybridization methods, enabling greater versatility and where possible field-enabled detection of target analytes. We present, herein, a hybrid molecular self-assembled scaffolded DNA origami entity, intimately immobilized via capture probes linked to aminopropyltriethoxysilane, onto a glass optical fiber end-face transducer, thus producing a novel biosensor. Immobilized DNA nanorobots with a switchable flap can then be actuated by a specific target DNA present in a sample, by exposing a hemin/G-quadruplex DNAzyme, which then catalyzes the generation of chemiluminescence, once the specific fiber probes are immersed in a luminol-based solution. Integrating organic nanorobots to inorganic fiber optics creates a hybrid system that we demonstrate as a proof-of-principle can be utilized in specific DNA sequence detection. This system has potential applications in a wide range of fields, including point-of-care diagnostics or cellular in vivo biosensing when using ultrathin fiber optic probes for research purposes.
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