期刊
BIOSENSORS & BIOELECTRONICS
卷 100, 期 -, 页码 274-281出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.09.022
关键词
Sulfur and nitrogen co-doped reduced graphene oxide; Tetrahedral DNA; Thrombin; Hybridization chain reaction; Aptasensor
类别
资金
- National Natural Science Foundation of China [21475115]
- Program for University Innovative Research Team of Henan [15IRTSTHN001]
- Henan Provincial Science and Technology Innovation Team [C20150026]
- XYNU
- Henan Science and Technology Cooperation Project [172106000064]
- Xinyang Normal University [2016KYJJ12]
A novel competitive aptasensor for thrombin detection is developed by using a tetrahedral DNA (T-DNA) probe and hybridization chain reaction (HCR) signal amplification. Sulfur and nitrogen co-doped reduced graphene oxide (SN-rGO) is firstly prepared by a simple reflux method and used for supporting substrate of biosensor. Then, T-DNA probe is modified on the electrode by Au-S bond and a competition is happened between target thrombin and the complementary DNA (cDNA) of aptamer. The aptamer binding to thrombin forms an aptamertarget conjugate and make the cDNA remained, and subsequently hybridizes with the vertical domain of T-DNA. Finally, the cDNAs trigger HCR, which results in a great current response by the catalysis of horseradish peroxidase to the hydrogen peroxide + hydroquinone system. For thrombin detection, the proposed biosensor shows a wide linearity range of 10(-13)-10(-8) M and a low detection limit of 11.6 fM (S/N = 3), which is hopeful to apply in biotechnology and clinical diagnosis.
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