期刊
BIOSENSORS & BIOELECTRONICS
卷 107, 期 -, 页码 153-162出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.02.026
关键词
Graphene quantum dots assembly; Alloy nanoparticles; Carbon fiber microelectrode; Electrochemical sensor; Cancer biomarker detection
类别
资金
- National Natural Science Foundation of China [51572094, 51504168]
- Innovation Research Funds of HUST [2017KFYXJJ164]
The development of high-efficient technologies for cancer biomarkers detection has attracted tremendous research effort for its great clinic significance. In this work, we designed a new type of flexible and robust nanohybrid microelectrode by modifying carbon fiber with dual nanoenzyme, Le., AuPd alloy nanoparticles (AuPdANPs) decorated graphene quantum dots (GQDs) assembly, and explored its practical application in electrochemical sensing system for sensitive detection of cancer biomarker hydrogen peroxide (H2O2) in human breast cancer cells and tissue. For the preparation of dual nanoenzyme modified microelectrode, ionic liquid was used as the electrolyte for the effective electrodeposition of GQDs on carbon fiber substrate to form a close-packed assembly under a very negative potential, then the highly dense AuPd-ANPs were uniformly decorated on GQDs assembly by electrodeposition. In virtue of the structural merits and synergistic contribution of dual nanoenzyme in enhancing the electrocatalytic activity to H2O2, the resultant nanohybrid microelectrode exhibited good sensing performances for electrochemical detection of H2O2, including a high sensitivity of 371 mu A cm(-2) mM(-1), a wide linear range from 1.0 mu M to 18.44 mM, a low detection limit of 500 nM (a signal-to-noise ratio of 3:1), as well as good selectivity and biocompatibility, which could be used for real-time tracking H2O2 released from different types of human breast cells and in situ sensitive detection of H2O2 in clinical breast cancer tissue.
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