期刊
BIOSENSORS & BIOELECTRONICS
卷 102, 期 -, 页码 321-327出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.11.043
关键词
Sandwich-type sensor; alpha 2,3-sialylated glycans; 4-mercaptophenylboronic acid; Maackia amurensis lectin; Methylene blue
类别
资金
- National Nature Science Foundation of China [81370403]
- Chongqing Foundation and Advanced Research Project [CSTC2015jcyjBX0053]
- Chongqing Precision Medical Key Technology Research and Development and Demonstration Projects [cstc2016shms-ztzx0042]
- Chongqing Medical University Scientific Research Cultivating Fund [201414]
alpha 2,3-sialylated glycans (alpha 2,3-sial-Gs) are one of the significant tumour biomarkers for the early diagnosis of cancer. In this work, a neoteric sandwich-type biosensor was developed for detecting alpha 2,3-sial-Gs using 4-mercaptophenylboronic acid (4-MPBA) to construct a novel molecular recognition system by the coordination of a boron atom of 4-MPBA to the amide group of Neu5Ac in the alpha 2,3-sial-Gs structure. Amino-functionalized fullerene coupled with palladium-platinum bimetallic alloy nanocrystals (n-C-60-PdPt) was synthesized to modify the surface of a glassy carbon electrode (GCE) because the n-C-60 nanomaterial affords a large surface area for the on-site reduction of bimetallic alloy nanoparticles and an excellent capacity for electron transfer. Abundant 4-MPBA were immobilized on the n-C-60-PdPt, since the 4-MPBA has the mercapto group can combine with PdPt alloy through strong adsorption. Maackia amurensis lectin (MAL) was covalently immobilized on Au-poly (methylene blue) (Au-PMB) acting as the signal amplification components, which was used to recognize the alpha 2, 3-sial-Gs specifically like a second antibody linked on Au-PMB. The differential pulse voltammetry (DPV) current response of the biosensor in 5 mL of PBS (0.1 M, pH = 7.4) was recorded, and the proposed sandwich-type biosensor showed a wide linear range of 10 fg mL(-1) -100 ng mL(-1) as well as, a low detection limit of 3 fg mL(-1) (S/N = 3). Furthermore, the proposed method exhibited good recovery and stability, indicating its potential for use in clinical studies.
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