期刊
BIOSENSORS & BIOELECTRONICS
卷 106, 期 -, 页码 204-211出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.01.062
关键词
Core-shell SERS nanotags; Lateral flow assay; Quantitative; Ultrasensitive; Multiplex detection; Acute myocardial infarction
类别
资金
- National Key Research and Development Program of China [2017YFA0205700]
- National Natural Science Foundation of China [21373046, 21327902, 61605082, 61675096]
- Program Sponsored for Scientific Innovation Research of College Graduate in Jiangsu Province [KYLX16_0284]
- Jiangsu Science and Technology Department [BE2014707]
- Natural Science Foundation of Jiangsu Province [BK20160969, BK2014021828]
- Fundamental Research Funds for the Central Universities
- Six Talent Peaks Project of Jiangsu Province
- Collaboration Research Fund of Southeast University
- Nanjing Medical University [2242017K3DN26]
- open research fund of Key Laboratory of Environmental Medicine Engineering of Ministry of Education
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [16KJB510020]
- China Postdoctoral Science Foundation [2017M611654]
- Jiangsu Postdoctoral Science Foundation [1701074B]
Rapid and sensitive quantification of multiplex proteins in a wide concentration range is challenging in high throughput analysis. Herein, we proposed a lateral flow assay (LFA) based on core-shell surface enhanced Raman scattering (SERS) nanotags for multiplex and quantitative detection of cardiac biomarkers for the early diagnosis of acute myocardial infarction (AMI). In practice, Raman dyes (RDs) were embedded into the interior-gap of silver core and gold shell nanoparticles (NPs) to form SERS nanotags as labels instead of gold colloids and three test lines were employed in the strip for the detection of three cardiac biomarkers, Myo, cTnI, and CK-MB, respectively. Due to the amplified signal of the SERS nanotags and the high surface area to volume ratio (SVR) of porous nitrocellulose (NC) membrane, ultrasensitive quantification of protein markers with wide linear dynamic range (LDR) was realized, which is crucial for the quick detection of multiplex biomarkers in the same sample without pretreatments at bedsides. This method makes it possible for LFA in point of care testing (POCT) to be comparable with chemiluminescence immunoassay (CLIA) used in labs.
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