期刊
BIOSENSORS & BIOELECTRONICS
卷 100, 期 -, 页码 41-48出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.08.057
关键词
Nitrogen-doped graphene quantum dot; Fluorescence enhancing; Al3+ detection; Cell imaging
类别
资金
- National Natural Science Foundation of China [81471697, 81771878]
- Key Technology R & D Program of Hubei Province [2014BBB003]
- Yellow Crane Talent (Science & Technology) Program of Wuhan City
- Applied Basic Research Program of Wuhan City [2016060101010044]
- Fundamental Research Funds for the Central Universities (Hust) [2016YXMS253]
Graphene quantum dot (GQD) has been attractive in analytical science field due to its low toxicity, stable photoluminescence. Herein, nitrogen-doped GQD (N-GQD) was prepared by a facile solvothermal treatment of GO using dimethylformamide, and exhibited a green emission with 23.1% quantum yield. The N-GQD probe showed a selective and sensitive fluorescence enhancement response to Al3+, the mechanism might be the formation of a complex between Al3+ and N-GQD constrained the photo-induced electron transfer (PET) process of N-GQD itself. With Benesi-Hildebrand equation, the binding constant and molar ratio between N-GQD and Al3+ was calculated to be 4.6 x 10(4) L mol(-1) and 1:1 respectively. The pK(a) value of N-GQD was also determined to be 4.4 by capillary electrophoresis. In pH 4.0 PBS solution, there was a good linear relation between the fluorescence intensity and the logarithm of concentration of Al3+ in the range of 2.5-75 mu mol L-1, the limit of detection (3 sigma) was 1.3 mu mol L-1. This Off - On fluorescence method had been applied to accurate quantification of aluminum in hydrotalcite tablets. What's more, the fluorescence switch property of N-GQD was explored by alternate addition of Al3+ and EDTA. The probe was also utilized for detection Al3+ in living cells due to its excellent biocompatibility.
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