期刊
BIOSENSORS & BIOELECTRONICS
卷 109, 期 -, 页码 272-278出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.03.002
关键词
alpha-hemolysin nanopore; Single-molecule detection; TPPS; Copper ion; Biosensor
类别
资金
- National Science Foundation of China [20175105, 21375104, 21327806]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20126101110015]
- Natural Science Basic Research Plan in Shaanxi Province of China [2016JQ2021]
- Shaanxi Provincial Education Department [16JK1780]
- Science Foundation of Northwest University [338020018]
It remains a significant challenge to study the interactions between metal ions and porphyrin molecules at single ion level. Here, we constructed a nanopore-based sensing for label-free and real-time analysis of the interaction between Cu2+ and 5,10,15,20-tetrakis(4-sulfonatophenyl)-porphyrin (TPPS). The results demonstrate that emerging electronic signatures of the Cu2+-TPPS complex that is completely different form the original free TPPS were observed in the alpha-hemolysin (alpha-HL) nanopore. Based on the distinctive electronic signal patterns between TPPS and Cu2+-TPPS complex, the unique nanopore sensor can achieve a highly sensitive detection of Cu2+ in aqueous media. The frequency of signature events showed a linear response toward the concentration of Cu2+ in the range of 0.03 mu M - 1.0 mu M, with a detection limit of 16 nM (S/N = 3). The sensing system also exhibited high selectivity against other metal ions, and the feasibility of this approach for practical applications was demonstrated with the determination of Cu2+ in running water.
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