期刊
BIOSENSORS & BIOELECTRONICS
卷 117, 期 -, 页码 145-152出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.06.006
关键词
CdZnTeS QDs; Immunosensors; Electrochemiluminescent image analysis; Alpha-fetoprotein; Cancer antigen 125
类别
资金
- National Key Research and Development Program of China [2017YFA0700404]
- National Natural Science Foundation of China [21575022, 21535003, 21375006]
- Fundamental Research Funds for the Central Universities [2242016K41055]
- Qing Lan Project
- Priority Academic Program Development of Jiangsu Higher Education Institutions [1107047002]
- Foundation of Key Laboratory of Sensor Analysis of Tumor Marker, MOE, Qingdao University of Science and Technology [STAM201801]
In this work, quaternary CdZnTeS quantum dots (QDs) with a particularly strong electrochemiluminescence (ECL) were synthesized as ECL signal labels. The strong ECL signals can be obtained at both cathode and anode with the ECL efficiencies of 19.78% and 1.62%, respectively. The sandwich-structured ECL immunosensors for the detection of alpha-fetoprotein (AFP) and cancer antigen 125 (CA125) were accomplished with direct ECL image analysis. Under optimal conditions, the QDs-based ECL image immunoanalysis possessed good linearity from 0.5 ng/mL to 20 ng/mL for AFP and from 20 U/mL to 500 U/mL for CA125 with the detection limit of 0.1 ng/mL and 6 U/mL, respectively (S/N = 3), and the lower detection limit obtained by photomultiplier tube were 0.1 fg/mL for AFP and 0.03 mU/mL for CA125 with the wide dynamic range from 0.5 fg/mL to 20 ng/mL and from 0.1 mU/mL to 500 U/mL, respectively (S/N = 3). Furthermore, the ECL immunoanalysis was evaluated with commercial enzyme-linked immunosorbent assay in human serum samples. The good results indicated that CdZnTeS QDs-based ECL biosensor has great potential for fast biomedical screening and multi assays in clinical diagnosis.
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