期刊
BIOSENSORS & BIOELECTRONICS
卷 117, 期 -, 页码 153-160出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.04.002
关键词
Point-of-care; Diagnostics; Multiplexed; Fluorescence; Colorimetry; Limit of detection
类别
资金
- National Science Foundation Smart and Connected Health [IIS-1521904]
- National Cancer Institute Cancer Detection, Diagnosis, and Treatment Technologies for Global Health [CA211415]
- Direct For Computer & Info Scie & Enginr
- Div Of Information & Intelligent Systems [1521904] Funding Source: National Science Foundation
An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3 x 3 mm interference filters with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters, we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of similar to 1 x 10(4) photons/mm(2) sec and has the potential for 2-3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing filter dimensions and ensuring appropriate channel isolation in a 2 x 2 array configuration. Practical considerations with low-cost interference filter system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.
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