期刊
BIOSENSORS & BIOELECTRONICS
卷 109, 期 -, 页码 13-19出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.02.062
关键词
Electrochemiluminescence biosensor; MIRNA-141; Faraday cage-type ECL biosensor; Hybridization chain reaction
类别
资金
- Science and Technology Department of Zhejiang Province of China [2016C33176]
- Natural Science Foundation of China [41576098, 81773483, 21605089]
- Ningbo Municipal Natural Science Foundation [2017A610228]
- Open Subject of State Key Laboratory of Chemo/Biosensing and Chemometrics [2016001]
- Project of Academic Research Foundation of Ningbo University [XYL17002]
- K.C. Wong Magna Fund in Ningbo University
In this study, a novel electrochemiluminescence (ECL) biosensor for sensitive detection of femtomolar miRNA-141 was constructed on the basis of Faraday cage-type strategy via graphene oxide (GO) and hybridization chain reaction (HCR)-assisted cascade amplification. A capture probe (CP) was immobilized on Fe3O4@SiO2@Au nanoparticles as capture unit, which could catch the miRNA-141, and the immobilization of the signal unit (Ru (phen)(3)(2+)-HCR/GO) was allowed via nucleic acid hybridization. The prepared biosensor exhibited two advantages for signal amplification: firstly, GO could lap on the electrode surface directly, extending Outer Helmholtz Plane (OHP) of the sensor due to the large surface area and good electronic transport property; secondly, HCR-assisted cascade amplification was designed by anchoring all HCR products on the GO surface, then embedding Ru(phen)(3)(2+) as a signal readout pathway. All these signal molecules could take part in electrochemical reactions, thus further enhancing the ECL signal drastically. Therefore, the proposed sensor constructed by integrating HCR with Faraday cage-type strategy displayed an ultrasensitive detection platform for the miRNA-141 with a low detection limit of 0.03 fM. In addition, this proposed biosensor provides a universal platform for analysis of other microRNAs.
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