期刊
BIOSENSORS & BIOELECTRONICS
卷 108, 期 -, 页码 69-75出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.02.043
关键词
Aptasensing device; Aflatoxin B1; Screen-printed carbon electrode; Label-free; Electrochemical impedance spectroscopy
类别
资金
- National Key Research and Development Program of China [2017YFD0400100]
- National Natural Science Foundation of China [21405063, 31671932, 21675066]
- Jiangsu Province Key Research and Development Plan (Modern Agriculture) [BE2015308]
- International Postdoctoral Exchange Fellowship Program of China Postdoctoral Council [20160015]
- China Postdoctoral Science Foundation [2015T80517]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [14KJA550001]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Aflatoxin B1 (AFB1), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this work, a magnetically assembled aptasensing device has been designed for label-free determination of AFB1 by employing a disposable screen-printed carbon electrode (SPCE) covered with a designed polydimethylsiloxane (PDMS) film as the micro electrolytic cell. The magnetically controlled bio-probes were firstly prepared by immobilization of the thiolated aptamers on the Fe3O4@Au magnetic beads, which was rapidly assembled on the working electrode of SPCE within 10 s, by using a magnet placed at the opposite side. The PDMS film with a centered hole was covered on the SPCE surface to achieve a more practicable and flexible electrochemical measurement. In this effort, a label-free aptasensor for the sensitive and selective determination of AFB1 has been developed using electrochemical impedance spectroscopy upon the biorecognition between aptamers and the targets. The developed method had a wide linear range of 20 pg mL(-1)-50 ng mL(-1) with a detection limit of 15 pg mL(-1) (S/N = 3) and succeeded in spiked samples of peanuts. The developed aptasensing device shows fantastic application prospect with simple design, easy operation, low cost, and high sensitivity and selectivity characteristics. This sensing strategy represents a promising path toward routine quality control of food safety and creates the opportunity to develop facile aptasensing device for other targets.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据