期刊
BIOSENSORS & BIOELECTRONICS
卷 99, 期 -, 页码 28-33出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.07.036
关键词
Graphene quantum dots; DNAzyme; Ionic liquid; Electrochemical aptasensor; Carcinoembryonic antigen
类别
资金
- National Natural Science Foundation of China [21405035, 21475032]
- Open Project Program of State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1501]
- Open Project Program of State Key Laboratory of Chemo/Biosensing and Chemometrics [Z2015021]
In this work, we have developed an electrochemical aptasensor for high-sensitivity determination of carcinoembryonic antigen (CEA) based on lead ion (Pb2+)-dependent DNAzyme-assisted signal amplification and graphene quantum dot-ionic liquid-nafion (GQDs-IL-NF) composite film. We designed hairpin DNA containing CEA-specific aptamers and DNAzyme chains. In the presence of CEA, hairpin DNA recognized the target and performed a DNAzyme-assisted signal amplification reaction to yield a large number of single stranded DNA. The GQDs-IL-NF composite film was immobilized on the glassy carbon electrode for the interaction with single-stranded DNA through noncovalent pi-pi stacking interaction. Therefore, the methylene blue-labeled substrate DNA (MB-substrate) was fixed on the electrode and exhibited an initial electrochemical signal. Under optimal conditions, the response current change was proportional to the concentration of CEA, demonstrating a wide linear range from 0.5 fg mL(-1) to 0.5 ng mL(-1), with a low detection limit of 0.34 fg mL(-1). Furthermore, the proposed aptasensor was successfully applied in determining CEA in serum samples, showing its superior prospects in clinical diagnosis.
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