期刊
BIOSENSORS & BIOELECTRONICS
卷 115, 期 -, 页码 30-36出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2018.05.026
关键词
Amperometric immunosensor; Polydopamine particles; Click reaction; Sandwich-type; Tumor marker
类别
资金
- National Natural Science Foundation of China [21273153, 21673143]
- Natural Science Foundation of Beijing Municipality [2172016, 2132008]
- High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [IDHT20180517]
- Capacity Building for Sci-Tech Innovation - Fundamental Scientific Research Funds [025185305000/195]
Sensitivity amplification strategy by implementing click chemistry in the construction of biosensing interface can efficiently improve the performance of immunosensor. Herein, we developed a sandwich-type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 24-2 (CA 242) based on pH responsive label-assisted click chemistry triggered sensitivity amplification strategy. The sensitivity of amperometric immunosensor relies on the current response differences (Delta I) caused by per unit concentration target analyte. The pH responsive Cu2+-loaded polydopamine (CuPDA) particles conjugated with detection antibodies were employed as labels, which can release Cu(II) ions by regulating pH. In the presence of ascorbic acid (reductant), Cu (II) ions were reduced to Cu(I) ions. Azide-functionalized double-stranded DNA (dsDNA) as signal enhancer was immobilized on the substrate through Cu+-catalyzed azide/alkyne cycloaddition reaction. With the help of the click reaction, the Delta I caused by target was elevated prominently, resulting in sensitivity amplification of the immunosensor. Under optimal condition, the proposed immunosensor exhibited excellent performance with linear range from 0.0001 to 100 U mL(-1) and ultralow detection limit of 20.74 mu U mL(-1). This work successfully combines click chemistry with pH-responsive labels in sandwich-type amperometric immunosensor, providing a promising sensitivity amplification strategy to construct immunosensing platform for analysis of other tumor marker.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据