期刊
BIOSENSORS & BIOELECTRONICS
卷 99, 期 -, 页码 519-524出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.08.028
关键词
Chemiluminescence; Luminol; N-Hydroxyphthalimide; Superoxide dismutase; Uric acid; Cobalt ions
类别
资金
- National Key Research and Development Program of China [2016YFA0201300]
- National Natural Science Foundation of China [21675148, 21475123]
- Chinese Academy of Sciences (CAS)-Academy of Sciences for the Developing World (TWAS) President's Fellowship Programme [2013-053]
N-hydroxyphthalimide (NHPI), a well known reagent in organic synthesis and biochemical applications, has been developed as a stable and efficient chemiluminescence coreactant for the first time. It reacts with luminol much faster than N-hydroxysuccinimide, eliminating the need of a prereaction coil used in N-hydroxysuccinimide system. Without using prereaction coil, the chemiluminescence peak intensities of luminol-NHPI system are about 102 and 26 times greater than that of luminol-N-hydroxysuccinimide system and classical luminol-hydrogen peroxide system, respectively. The luminol-NHPI system achieves the highly sensitive detection of luminol (LOD = 70 pM) and NHPI (LOD = 910 nM). Based on their excellent quenching efficiencies, superoxide dismutase and uric acid are sensitively detected with LODs of 3 ng/mL and 10 pM, respectively. Co2+ is also detected a LOD of 30 pM by its remarkable enhancing effect. Noteworthily, our method is at least 4 orders of magnitude more sensitive than previously reported uric acid detection methods, and can detect uric acid in human urine and Co2+ in tap and lake water real samples with excellent recoveries in the range of 96.35-102.70%. This luminol-NHPI system can be an important candidate for biochemical, clinical and environmental analysis.
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