期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 82, 期 7, 页码 1116-1122出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2018.1456316
关键词
Gallbladder carcinoma; RNA interference; heparanase; tumor invasion and metastasis
This study investigated the effect of transcriptional gene silencing of the heparanase gene on standard gallbladder carcinoma cells (GBC-SD). The miRNAs targeting the promoter region and coding region of the heparanase gene were designed and synthesized. We transfected four recombinant miRNA vectors into GBC-SD. We performed the wound healing assays and invasion assays. The result shows that the heparanase expression was significantly decreased by recombinant vectors in transfected GBC-SD cells (p<0.01), of which pmiR-Hpa-2 showed best interference effect (p<0.05). The penetrated and migrating cells numbers and adherence rate of GBC-SD cells were significantly decreased by pmiR-Hpa-2 (p<0.05).
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